Sensor Arrays on a Centrifugal Platform for Bacterial Identification and Quantification (ID/Q) and Antibiotics Susceptibility Testing (AST)

Marc J. Madou

Distinguished Professor, Mechanical and Aerospace Engineering, UC Irvine

Seminar Information

Seminar Series
Biomechanics & Medical Devices

Seminar Date - Time
April 30, 2021, 9:00 am
-
10:00

Seminar Location
Zoom Meeting ID: 962 0467 5174
Password: Euler

Recording is available. Please contact Lusia at lveksler@ucsd.edu


Abstract

Rapid, point-of-care (PoC) diagnostics for antibiotic susceptibility testing (AST) are critical in combating the antimicrobial resistance epidemic. While new, alternative technologies are capable of rapidly identifying antibiotic resistance, traditional AST methods, where a patient sample is incubated with different antibiotics, remain the most reliable and practical in determining antibiotic effectiveness. Here, we demonstrate a novel sample incubation technique on a microfluidic centrifugal disc (CD) as a proof of concept automated sample processing platform for AST. By using ribosomal RNA (rRNA) as a marker for cell growth, we demonstrated that incubation on a microfluidic CD was enhanced (>1.6 fold) for 11 out of 14 clinically relevant isolates of Escherichia coli compared to traditional shaker incubators. Finally, we utilize the system to identify antibiotic resistance of 11 E. coli isolates incubated with 5 different antibiotics in under 2 hours.

Speaker Bio

Dr. Mark Madou - Distinguished Professor in the Department of Mechanical and Aerospace Engineering at UCI, NAI Fellow, Foreign Member Academia Mexicana de Ciencias.
Before joining UCI as the Chancellor’s Professor in Mechanical and Aerospace Engineering (MEA), Dr. Madou was Vice President of Advanced Technology at Nanogen in San Diego. Madou was the founder of SRI International’s Microsensor Department, founder and President of Teknekron Sensor Development Corporation (TSDC), Visiting Miller Professor at UC Berkeley and Endowed Chair at the Ohio State University (Professor in Chemistry and Materials Science and Engineering). He specializes in the
application of miniaturization technology to chemical and biological problems (BIO-MEMS). He is the author of several books in this burgeoning field he helped pioneer both in Academia and in Industry. “Fundamentals of Microfabrication,” an introduction to MEMS and NEMS, has become known as the “bible” of micromachining. He founded several micromachining companies and has been on the board of many more. Many of his students became well known in their own right in academia and through
successful MEMS start-ups. Dr. Madou, today, works with research teams in India (IIT Kharagpur), Mexico (Tec de Monterrey and UNAM), Malaysia (UM) and Germany (KIT). In the recent past he also worked on large projects with teams in South-Korea and Canada. Dr. Madou has an h-index of 78 (as of April 6, 2021) and he is considered the pioneer of two research fields that are now being pursued worldwide i.e., Carbon Micro- and Nanofabrication (C-MEMS and C- NEMS) and Compact Disc Fluidics (CD-Fluidics) for Molecular Diagnostics. These two technologies have resulted in at least 10 start-up companies worldwide. From those founded by Dr. Madou, Enevate, a
lithium- ion battery company in Irvine, CA) is the largest and best known.

To find out more about his most recent research projects, visit www.biomems.net